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five–four μmol/L vary,[156][157] the focus of serotonin in synaptic vesicles was calculated at 270 mM.[182] Following vesicular release, the resulting focus of serotonin in the synaptic cleft, to which serotonin receptors are exposed, is approximated to be about three hundred μmol/L. Consequently, when in vitro receptor binding affinities, ef
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